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Characterization and Validation of Cre-Driver Mouse Lines.

Françoise Gofflot^1,2, Olivia Wendling^1,3, Nathalie Chartoire¹

¹Institut Clinique de la Souris (ICS), Illkirch, France.

Current Protocols in Mouse Biology

|June 13, 2015

Summary

Researchers need to validate Cre transgenic mouse lines for reliable gene manipulation in biomedical research. This overview discusses essential validation information and methods for Cre recombinase expression and activity.

Keywords:

conditional mutagenesis functional genomic inducible Cre site-specific recombination

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Area of Science:

Genetics
Mouse Models
Biomedical Research

Background:

Conditional gene manipulations using genetically engineered mutant mouse (GEMM) lines are crucial in biomedical research.
These approaches require breeding GEMMs with transgenic mouse lines expressing site-specific recombinases, primarily Cre recombinase, in a tissue-specific manner.

Purpose of the Study:

To outline the minimum information required for validating Cre-driver transgenic mouse lines.
To address challenges in validation procedures and propose solutions for assessing Cre recombinase expression and activity.

Main Methods:

Review of validation requirements for Cre-driver transgenic lines.
Discussion of methods to assess Cre expression and recombination activity.
Consideration of constitutive and inducible Cre recombinase systems.

Main Results:

Identification of essential data for validating Cre-driver lines.
Proposed strategies to overcome validation difficulties.
Methods to evaluate Cre recombinase function before extensive breeding.

Conclusions:

Rigorous validation of Cre-driver transgenic lines is critical for successful conditional gene manipulation.
Standardized validation protocols ensure reliable and efficient generation of somatic mutations in research models.
Assessing Cre recombinase expression and activity early prevents resource waste in phenotyping studies.