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Updated: Apr 10, 2026

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

Published on: October 7, 2018

Lentiviral Vector Mediated Transgenesis.

Isabelle Barde¹, Sonia Verp¹, Sandra Offner¹

¹School of Life Sciences and "Frontiers in Genetics" National Program, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.

Current Protocols in Mouse Biology

|June 13, 2015

Summary

This summary is machine-generated.

Lentiviral vectors offer a simple, efficient, and cost-effective method for creating transgenic animals, advancing biomedical research and disease modeling. This technique surpasses traditional methods for genetic manipulation in rodents.

Keywords:

lentiviral vector transgenesis transgenic animals

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Last Updated: Apr 10, 2026

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Laser-assisted Lentiviral Gene Delivery to Mouse Fertilized Eggs

Laser-assisted Lentiviral Gene Delivery to Mouse Fertilized Eggs

Published on: November 1, 2018

Area of Science:

Biomedical Research
Genetics
Molecular Biology

Background:

Genetic manipulation of rodents is crucial for biomedical research and developing disease models.
Traditional methods like pronuclear injection are established but have limitations.

Purpose of the Study:

To highlight the utility of lentiviral vectors for generating transgenic animals.
To compare lentiviral vector-mediated transgenesis with existing techniques.

Main Methods:

Utilizing lentiviral vectors for gene delivery and stable transgene expression.
Application in various cell types, including stem cells, and direct in vivo administration.
Leveraging diverse lentiviral vector designs for controlled gene expression.

Main Results:

Lentiviral vectors enable efficient and stable integration of transgenes both in vitro and in vivo.
This method is simpler, cheaper, and more efficient than pronuclear injection for creating transgenic rodents.
Allows for versatile transgene expression patterns (ubiquitous, tissue-specific, controllable) and gene knockdown.

Conclusions:

Lentiviral vector-mediated transgenesis represents a significant advancement in creating genetically modified animal models.
Offers enhanced flexibility and efficiency for fundamental research and preclinical studies.
Facilitates the development of sophisticated genetic tools for diverse research applications.