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Related Experiment Video

Updated: Apr 10, 2026

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Published on: June 15, 2017

Generating and Analyzing Germ-Free Mice.

Carina Arvidsson¹, Anna Hallén¹, Fredrik Bäckhed¹

¹Department of Molecular and Clinical Medicine, Sahlgrenska Center for Cardiovascular and Metabolic Research/Wallenberg Laboratory, University of Gothenburg, Gothenburg, Sweden.

Current Protocols in Mouse Biology

|June 13, 2015

Summary

This summary is machine-generated.

This study provides essential protocols for creating and maintaining germ-free mice, crucial for understanding how the gut microbiota influences host biology, immunity, and diseases like obesity.

Keywords:

gnotobiotic mouse models gut microbiota hysterectomy

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Area of Science:

Microbiology
Animal Science
Physiology

Background:

The gut microbiota significantly impacts host biology, including immune function, metabolism, and behavior.
Germ-free animal models are vital for dissecting the specific roles of the gut microbiota.
Altered gut microbiota is linked to conditions such as obesity.

Purpose of the Study:

To present detailed protocols for the generation and maintenance of germ-free mice.
To facilitate research into the host-microbiota relationship.

Main Methods:

Detailed step-by-step protocols for establishing germ-free conditions in mice.
Methods for maintaining the health and integrity of germ-free animal colonies.

Main Results:

Successful generation and maintenance of germ-free mice are achievable with the presented protocols.
These protocols enable rigorous investigation into microbiota-dependent physiological processes.

Conclusions:

Germ-free mouse models are indispensable tools for microbiome research.
Standardized protocols are essential for reproducible and reliable studies on gut microbiota and host interactions.