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Updated: Apr 10, 2026
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High-resolution Respirometry to Assess Mitochondrial Function in Permeabilized and Intact Cells
Published on: February 8, 2017
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High-Resolution Respirometry for Mitochondrial Characterization of Ex Vivo Mouse Tissues
Carles Cantó1, Pablo M Garcia-Roves2
1Nestlé Institute of Health Sciences S.A, Lausanne, Switzerland.
Current Protocols in Mouse Biology
|June 13, 2015
Abstract:
This article describes methodologies to examine mitochondrial respiration in fresh preparations of mouse tissues, including skeletal muscle, heart, liver, white and brown adipose tissue, and brain. Reference values and tips to maximize experimental efficiencies are also provided. Finally, correction methods and complementary techniques to properly interpret the results are presented and contrasted.
