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Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice
Published on: February 3, 2023
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Phenotypic Analysis of BAT versus WAT Differentiation.
Matthias Rosenwald1, Aliki Perdikari1, Elisabeth Weber1
1Institute of Food, Nutrition, and Health, ETH Zurich, Schwerzenbach, Switzerland.
Current Protocols in Mouse Biology
|June 13, 2015
Summary
Obesity is a growing epidemic linked to serious health issues. This study presents new methods for analyzing white and brown adipose tissue, crucial for understanding energy balance and metabolism.
Area of Science:
- Metabolic research
- Cell biology
- Obesity research
Background:
- The Western world faces an obesity epidemic, leading to severe complications like type 2 diabetes, hypertension, and cardiovascular disease.
- Obesity results from a chronic energy imbalance, where energy intake surpasses expenditure.
- White adipose tissue (WAT) stores energy, while brown adipose tissue (BAT) regulates metabolism and body temperature.
Purpose of the Study:
- To describe novel approaches for analyzing white adipose tissue (WAT) and brown adipose tissue (BAT).
- To overcome the challenges associated with adipose tissue analysis, such as high triglyceride content and cell fragility.
Main Methods:
- Detailed protocols for the isolation and analysis of WAT and BAT.
- Techniques designed to preserve tissue integrity and cellular components during sample preparation.
Main Results:
- Successful implementation of methods allowing for detailed cellular and molecular analysis of adipose tissues.
- Demonstration of techniques that mitigate common difficulties in working with adipose tissue.
Conclusions:
- The described methods provide reliable tools for researchers studying adipose tissue.
- These advancements facilitate a deeper understanding of obesity and metabolic homeostasis.
![Visualization and Quantification of Brown and Beige Adipose Tissues in Mice using [18F]FDG Micro-PET/MR Imaging](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F62460.jpg&w=3840&q=50)