Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

6.0K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
6.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Major improvement using peroperative 3D compared to 2D imaging for SI screw fixation in traumatic pelvic fractures.

European journal of trauma and emergency surgery : official publication of the European Trauma Society·2026
Same author

Physeal Allograft Transfer for Physeal Bars: A Safety and Feasibility Study in a Domestic Swine Model.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society·2026
Same author

Local delivery of lipid-based nanoparticles containing microbial nucleic acid for osteoimmunomodulation.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences·2025
Same author

Trained innate immunity modulates osteoblast and osteoclast differentiation.

Stem cell reviews and reports·2024
Same author

Prediction of continuous and discrete kinetic parameters in horses from inertial measurement units data using recurrent artificial neural networks.

Scientific reports·2023
Same author

Sustained release of locally delivered celecoxib provides pain relief for osteoarthritis: a proof of concept in dog patients.

Osteoarthritis and cartilage·2022

Related Experiment Video

Updated: Apr 10, 2026

Fabrication of Decellularized Cartilage-derived Matrix Scaffolds
08:02

Fabrication of Decellularized Cartilage-derived Matrix Scaffolds

Published on: January 7, 2019

11.6K

Multipotent Stromal Cells Outperform Chondrocytes on Cartilage-Derived Matrix Scaffolds.

K E M Benders1, W Boot1, S M Cokelaere2

  • 1Department of Orthopedics, University Medical Center Utrecht, Utrecht, the Netherlands.

Cartilage
|June 13, 2015
PubMed
Summary

Cartilage-derived matrix (CDM) scaffolds show potential for repairing cartilage defects. Multipotent stromal cells (MSCs) seeded on CDM demonstrated superior chondrogenic differentiation compared to chondrocytes in vitro.

Keywords:
cartilage tissue engineeringchondrocyteextracellular matrix (ECM)mesenchymal stromal cellscaffold

More Related Videos

Chondrogenic Pellet Formation from Cord Blood-derived Induced Pluripotent Stem Cells
12:10

Chondrogenic Pellet Formation from Cord Blood-derived Induced Pluripotent Stem Cells

Published on: June 19, 2017

11.8K
Isolation of Chondrocytes and Chondroprogenitors Using Fibronectin Adhesion and Migratory Assay
08:09

Isolation of Chondrocytes and Chondroprogenitors Using Fibronectin Adhesion and Migratory Assay

Published on: October 4, 2024

1.4K

Related Experiment Videos

Last Updated: Apr 10, 2026

Fabrication of Decellularized Cartilage-derived Matrix Scaffolds
08:02

Fabrication of Decellularized Cartilage-derived Matrix Scaffolds

Published on: January 7, 2019

11.6K
Chondrogenic Pellet Formation from Cord Blood-derived Induced Pluripotent Stem Cells
12:10

Chondrogenic Pellet Formation from Cord Blood-derived Induced Pluripotent Stem Cells

Published on: June 19, 2017

11.8K
Isolation of Chondrocytes and Chondroprogenitors Using Fibronectin Adhesion and Migratory Assay
08:09

Isolation of Chondrocytes and Chondroprogenitors Using Fibronectin Adhesion and Migratory Assay

Published on: October 4, 2024

1.4K

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Extracellular matrix (ECM)-derived scaffolds are widely used in clinical applications.
  • The application of ECM as a biomaterial for osteochondral regeneration remains underexplored.
  • Cartilage-derived matrix (CDM) scaffolds offer a promising avenue for cartilage repair.

Purpose of the Study:

  • To evaluate the chondrogenic potential of cells seeded on CDM scaffolds in vitro.
  • To compare the efficacy of chondrocytes and multipotent stromal cells (MSCs) in regenerating cartilage using CDM scaffolds.

Main Methods:

  • CDM scaffolds were created from decellularized equine articular cartilage.
  • Scaffolds were seeded with either chondrocytes or MSCs.
  • Constructs were cultured in vitro for 2, 4, and 6 weeks and analyzed histologically and biochemically for glycosaminoglycan (GAG) and DNA content.

Main Results:

  • Both chondrocytes and MSCs exhibited chondrogenic differentiation on CDM scaffolds after 4 weeks.
  • MSCs significantly outperformed chondrocytes in producing new GAG-containing cartilaginous matrix.
  • Histological and biochemical analyses confirmed matrix deposition and cell viability.

Conclusions:

  • CDM scaffolds demonstrate significant potential for osteochondral defect repair.
  • MSCs show superior chondrogenic capacity compared to chondrocytes when cultured on CDM scaffolds.
  • These findings support the use of CDM scaffolds in regenerative medicine for cartilage repair.