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Related Experiment Videos

Lymphokine-activated killer cell activity after cryopreservation.

I G Schmidt-Wolf1, M Aihara, R S Negrin

  • 1Division of Hematology, Stanford University, CA 94305.

Journal of Immunological Methods
|December 20, 1989
PubMed
Summary

Cryopreservation reduces the effectiveness of lymphokine-activated killer (LAK) cells, but their cytotoxic activity can be restored. Re-incubation with interleukin-2 (IL-2) after thawing preserves LAK cell function for immunotherapy.

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Area of Science:

  • Immunology
  • Cell Biology
  • Cryobiology

Background:

  • Lymphokine-activated killer (LAK) cells are crucial for adoptive immunotherapy.
  • Cryopreservation is a standard method for preserving biological materials, but its impact on LAK cell function requires investigation.

Purpose of the Study:

  • To evaluate the effect of cryopreservation on the cytotoxic activity of LAK cells.
  • To determine methods for restoring the function of cryopreserved LAK cells.

Main Methods:

  • LAK cells were generated from peripheral blood lymphocytes incubated with recombinant interleukin-2 (rIL-2).
  • Cells were cryopreserved using a programmed freezer and assessed for cytotoxicity via a 51Cr release assay.
  • Post-thaw cytotoxicity was measured before and after reincubation with rIL-2.

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Main Results:

  • Cryopreservation led to a significant reduction in LAK cell cytotoxic activity (25.5-39.1% decrease).
  • Reincubation with rIL-2 for 2 days post-thaw restored cytotoxicity to pre-cryopreservation levels.
  • Restored cytotoxicity was comparable to that of fresh LAK cells.

Conclusions:

  • Cryopreservation diminishes LAK cell cytotoxic function.
  • A two-step incubation process with rIL-2 (pre- and post-cryopreservation) maximizes the efficacy of cryopreserved LAK cells.
  • Cryopreserved LAK cells hold potential for clinical applications in adoptive immunotherapy.