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Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Preparation and Testing of Impedance-based Fluidic Biochips with RTgill-W1 Cells for Rapid Evaluation of Drinking Water Samples for Toxicity

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Detecting total toxicity in water using a mediated biosensor system with flow injection.

Daming Yong¹, Changyu Liu¹, Chengzhou Zhu¹

¹State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.

|June 15, 2015

Summary

A new mediated biosensor with flow injection (MB-FI) offers rapid water toxicity detection. This biosensor system reliably identifies respiration inhibitors, simplifying testing and enabling commercialization.

Keywords:

Flow injection mode Immobilized microorganism Mediated biosensor Total toxicity

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A Whole Cell Bioreporter Approach to Assess Transport and Bioavailability of Organic Contaminants in Water Unsaturated Systems

A Whole Cell Bioreporter Approach to Assess Transport and Bioavailability of Organic Contaminants in Water Unsaturated Systems

Published on: December 24, 2014

Area of Science:

Environmental Science
Analytical Chemistry
Biotechnology

Background:

Water toxicity detection is crucial for environmental safety.
Existing methods for detecting respiration inhibitors can be time-consuming.
A need exists for rapid, reliable, and simplified toxicity assessment tools.

Purpose of the Study:

To develop and validate a novel mediated biosensor system with flow injection (MB-FI) for detecting specific waterborne respiration inhibitors.
To assess the efficiency and reliability of the MB-FI system compared to classic methods.
To facilitate the application and commercialization of advanced toxicity monitoring technology.

Main Methods:

Utilized a mediated biosensor system with immobilized microorganisms in calcium alginate filaments.
Employed flow injection (FI) for rapid sample introduction and analysis.
Measured the inhibition of microbial respiration by injected compounds (As2O3, KCN, SA, DNP) using ferricyanide/ferrocyanide redox couple.
Determined the 50% inhibition concentration (IC50) as the toxicity metric.

Main Results:

The MB-FI system successfully detected four common respiration inhibitors: As2O3, KCN, salicylic acid (SA), and 2,4-dinitrophenol (DNP).
IC50 values obtained using the MB-FI method were comparable to those from classic toxicity testing methods.
The biosensor system demonstrated a simplified testing process and reduced analysis time.

Conclusions:

The developed MB-FI method is a viable and alternative alert system for rapid water toxicity monitoring.
The immobilized microorganism biosensor simplifies the testing procedure and saves time.
The MB-FI system shows significant potential for practical application and commercialization in toxicity monitoring.