Use of GLV-1h68 for Vaccinia Virotherapy and Monitoring
Alexa Frentzen1, Ulrike Geissinger, Desislava Tsoneva
1Genelux Corporation, 3030 Bunker Hill St, San Diego, CA, 92109, USA.
Methods in Molecular Biology (Clifton, N.J.)
|June 15, 2015
Abstract:
Herein we describe the use of the vaccinia virus strain GLV-1h68 as a theragnostic agent in cancer models. To date, GLV-1h68 has been used successfully in more than 50 xenograft tumor models. The recombinant vaccinia virus strain has been equipped with heterologous expression cassettes for a luciferase-fluorescent protein fusion gene, bacterial beta-galactosidase, as well as a bacterial glucuronidase. The methods to investigate and monitor GLV-1h68 mediated virotherapy, including optical imaging and biomarker analysis, will be presented in detail.
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