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Direct wavefront sensing for high-resolution in vivo imaging in scattering tissue
Kai Wang1, Wenzhi Sun1, Christopher T Richie2
1Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, Virginia 20147, USA.
Nature Communications
|June 16, 2015
Summary
Adaptive optics imaging compensates for light scattering in tissues using near-infrared guide stars. This novel approach enables deep in vivo brain imaging in mice, advancing neuroscience research.
Area of Science:
- Biomedical Optics
- Neuroimaging
- Adaptive Optics
Background:
- Adaptive optics (AO) corrects wavefront distortions for improved imaging.
- Current AO methods are limited in scattering tissues like biological tissue.
- Near-infrared (NIR) light scatters less in tissue than visible light.
Purpose of the Study:
- To extend adaptive optics imaging to strongly scattering biological tissues.
- To enable deep in vivo brain imaging using NIR guide stars.
Main Methods:
- Developed an AO system utilizing NIR guide stars to overcome scattering in biological tissues.
- Implemented direct wavefront sensing of NIR guide stars.
- Performed in vivo two-photon microscopy in mouse brains.
Main Results:
- Successfully compensated for aberrations caused by light scattering in mouse brain tissue.
- Achieved in vivo two-photon imaging at depths up to 700 μm.
- Demonstrated morphological and functional imaging capabilities within the mouse brain.
Conclusions:
- Adaptive optics with NIR guide stars is effective for deep tissue imaging.
- This technique significantly advances in vivo neuroimaging capabilities.
- Opens new avenues for studying brain structure and function in scattering tissues.
