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A comparison of reference method values for calcium, lithium and magnesium with method-dependent assigned values.

W R Külpmann1, R Buchholz, C Dyrssen

  • 1Institut für Klinische Chemie I, Medizinische Hochschule Hannover.

Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie
|September 1, 1989
PubMed
Summary

Routine methods for analyzing calcium, lithium, and magnesium in control sera showed varying accuracy. Flame atomic absorption spectrometry (FAAS) generally agreed well with reference methods, while other colorimetric methods for magnesium were less suitable.

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Area of Science:

  • Clinical Chemistry
  • Analytical Chemistry
  • Biochemistry

Background:

  • Accurate measurement of electrolytes like calcium, lithium, and magnesium is crucial for clinical diagnostics.
  • Commercially available control sera are essential for quality assessment of laboratory testing.
  • Evaluating the performance of routine analytical methods against reference standards is vital for ensuring reliable patient results.

Purpose of the Study:

  • To assess the accuracy of routine analytical methods for calcium, lithium, and magnesium in control sera.
  • To compare the performance of different analytical techniques, including flame atomic absorption spectrometry (FAAS) and colorimetric assays, against established reference methods.
  • To determine the suitability of routine methods for quality assessment requirements in clinical laboratories.

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Main Methods:

  • Analysis of commercially available control sera using reference methods for calcium, lithium, and magnesium.
  • Comparison of target values from routine methods (FAAS, flame atomic emission spectrometry, methylthymol blue, cresolphthalein, Magon, Calmagite) with reference method values.
  • Calculation of mean deviations and biases to quantify method performance.

Main Results:

  • Calcium: Routine FAAS showed a -1.2% deviation, flame atomic emission spectrometry -0.1%, while methylthymol blue and cresolphthalein assays exhibited positive biases (+2.3% and +2.2%).
  • Lithium: Routine FAAS and flame atomic emission spectrometry showed good agreement with reference methods (+1.7% and +0.7% deviation), though concentration-dependent differences were observed.
  • Magnesium: Routine FAAS had a low bias (-0.4%), but colorimetric methods (Magon, Calmagite) showed significant positive biases (+8.2%, +9.3%) and were deemed less suitable, potentially failing quality assessment.

Conclusions:

  • Flame atomic absorption spectrometry (FAAS) demonstrates good accuracy for routine analysis of calcium, lithium, and magnesium in control sera when compared to reference methods.
  • Colorimetric methods for magnesium determination (Magon, Calmagite) exhibit considerable bias and are less suitable for clinical laboratory quality assessment compared to FAAS.
  • The findings highlight the importance of method selection and validation to ensure reliable electrolyte measurements in patient samples.