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Author Spotlight: Unveiling Mitochondrial Function and Cellular Metabolic Adaptation in Metabolic Diseases
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Oxidative proteome alterations during skeletal muscle ageing.
Sofia Lourenço Dos Santos1, Martin A Baraibar1, Staffan Lundberg2
1Sorbonne Universités, UPMC Univ Paris 06, UMR 8256, Biological Adaptation and Ageing-IBPS, Paris F-75005, France; CNRS UMR-8256, Paris F-75005, France; Inserm U1164, Paris F-75005, France.
Redox Biology
|June 16, 2015
Summary
Oxidative stress damages proteins in aging skeletal muscle, contributing to sarcopenia. Identifying these oxidized proteins offers insights into muscle aging mechanisms and potential therapeutic targets.
Area of Science:
- Gerontology
- Molecular Biology
- Muscle Physiology
Background:
- Sarcopenia, the age-related loss of muscle mass and strength, significantly impairs mobility and quality of life.
- Cellular aging is characterized by accumulating oxidatively modified proteins, potentially disrupting cellular functions.
- While oxidative stress is implicated in muscle aging, specific oxidized protein targets remain unidentified.
Purpose of the Study:
- To identify specific oxidized proteins in human skeletal muscle associated with aging.
- To investigate the functional implications of protein carbonylation in the context of sarcopenia.
Main Methods:
- Proteomic analysis using two-dimensional gel electrophoresis.
- Immunodetection of carbonylated proteins in human rectus abdominis muscle biopsies from young and old donors.
Main Results:
- Seventeen protein spots showed increased carbonylation in older donors compared to younger donors.
- These identified proteins are crucial for cellular morphology, transport, muscle contraction, and energy metabolism.
- Oxidative modification of these proteins may impair key skeletal muscle functions.
Conclusions:
- Protein carbonylation is a significant molecular mechanism contributing to sarcopenia.
- The identified oxidized proteins represent potential targets for understanding and mitigating age-related muscle decline.
Keywords:
AgeingHuman rectus abdominis skeletal muscleOxidative stressProtein carbonylationProteomicsMore Related Videos
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