Related Experiment Video
Updated: Apr 10, 2026
Assays for the Identification of Novel Antivirals against Bluetongue Virus
Published on: October 11, 2013
Development of reverse transcription loop mediated isothermal amplification assay for rapid detection of bluetongue
Sreekala S Mohandas1, Dhanavelu Muthuchelvan1, Awadh Bihari Pandey1
1Division of Virology, Indian Veterinary Research Institute, Mukteswar, Uttarakhand 263 138, India.
Abstract:
A single-step reverse transcription loop mediated isothermal amplification (RT-LAMP) assay targeting NS1 - a highly conserved gene among BTV serotypes was optimized and validated with seven serotypes: BTV-1, BTV-2, BTV-9, BTV-10, BTV-16, BTV-21 and BTV-23. The relative sensitivity of the assay was 0.3 TCID50 and no cross reactivity could be observed with foot and mouth disease, peste-des-petits-ruminants, goatpox, sheeppox and orf viruses. The established assay was also assessed by screening of clinical samples and the result is comparable with conventional RT-PCR. The RT-LAMP assay described here could be an additional tool to the existing assays for diagnosis/surveillance of BTV.
