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    Area of Science:

    • Biomedical Optics
    • Optical Metrology
    • Birefringence Imaging

    Background:

    • Polarization-sensitive optical coherence tomography (PS-OCT) is crucial for characterizing birefringent tissues.
    • Existing PS-OCT methods often face challenges with speed, complexity, or accuracy.

    Purpose of the Study:

    • To develop and validate a new, efficient PS-OCT strategy for quantitative birefringence measurement.
    • To enable simultaneous acquisition of polarization state information from a single spatial region.

    Main Methods:

    • Utilized orthogonally polarized optical frequency combs (OFCs) in the sample arm of a single-mode fiber system.
    • Interleaved OFCs in the spectral domain for simultaneous measurement of both polarization states.
    • Employed wavelength stabilization and Jones matrix methods for quantitative phase retardation analysis.

    Main Results:

    • Successfully demultiplexed and analyzed orthogonally polarized states using interpolation after wavelength stabilization.
    • Validated birefringence measurement accuracy using a glass plate and quarter-wave plate.
    • Demonstrated the system's potential in a biomedical context by measuring chicken breast muscle birefringence.

    Conclusions:

    • The proposed PS-OCT strategy offers a robust method for quantitative birefringence imaging.
    • Simultaneous measurement of polarization states enhances efficiency and accuracy.
    • This technique holds promise for advanced biomedical imaging and diagnostics.