Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nucleosome Remodeling02:54

Nucleosome Remodeling

11.7K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
11.7K
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

2.3K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
2.3K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

10.0K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
10.0K
Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

7.6K
The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
7.6K
Euchromatin01:01

Euchromatin

9.3K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
9.3K
Heterochromatin02:38

Heterochromatin

19.1K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
19.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SARS-CoV-2 nucleocapsid protein variants have differential RNA chaperone activity.

The FEBS journal·2025
Same author

Adenovirus maturation establishes the transcription competent packaging of its genome.

EMBO reports·2025
Same author

Transcription-replication conflicts drive R-loop-dependent nucleosome eviction and require DOT1L activity for transcription recovery.

Nucleic acids research·2025
Same author

Plasmodium blood stage development requires the chromatin remodeller Snf2L.

Nature·2025
Same author

nucMACC: An MNase-seq pipeline to identify structurally altered nucleosomes in the genome.

Science advances·2024
Same author

The Long Non-Coding RNA MALAT1 Modulates NR4A1 Expression through a Downstream Regulatory Element in Specific Cancer Cell Types.

International journal of molecular sciences·2024

Related Experiment Video

Updated: Apr 10, 2026

Biochemical Assays for Analyzing Activities of ATP-dependent Chromatin Remodeling Enzymes
10:14

Biochemical Assays for Analyzing Activities of ATP-dependent Chromatin Remodeling Enzymes

Published on: October 25, 2014

15.4K

Chromatin Remodelers: From Function to Dysfunction.

Gernot Längst1, Laura Manelyte2

  • 1Biochemistry Center Regensburg, Laboratory of Chromatin Dynamics and Nuclear Architecture, University of Regensbrug, Universitätstraße 31, Regensburg DE-93053, Germany. Gernot.Laengst@vkl.uni-regensburg.de.

Genes
|June 16, 2015
PubMed
Summary

Chromatin remodelers regulate DNA accessibility and are crucial for cellular processes. Their deregulation can lead to cancer, highlighting the importance of their precise targeting via interactions with signals like non-coding RNAs.

Keywords:
arrest modelcancerchromatin remodelernon-coding RNAsearch mechanism

More Related Videos

Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes
08:44

Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes

Published on: October 23, 2014

10.4K
Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

7.1K

Related Experiment Videos

Last Updated: Apr 10, 2026

Biochemical Assays for Analyzing Activities of ATP-dependent Chromatin Remodeling Enzymes
10:14

Biochemical Assays for Analyzing Activities of ATP-dependent Chromatin Remodeling Enzymes

Published on: October 25, 2014

15.4K
Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes
08:44

Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes

Published on: October 23, 2014

10.4K
Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

7.1K

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Cancer Biology

Background:

  • Chromatin remodelers are essential for DNA-dependent biological processes by controlling chromatin accessibility and nucleosome positioning.
  • Deregulation of chromatin remodeling complexes can contribute to the development of cancer.
  • Effective targeting of specific chromatin remodelers to DNA is critical for proper gene regulation.

Purpose of the Study:

  • To review the mechanisms of chromatin remodeler targeting to chromatin.
  • To summarize the role of chromatin remodeling in cancer development.
  • To highlight the emerging importance of non-coding RNAs in guiding chromatin remodelers.

Main Methods:

  • Literature review of current research on chromatin remodelers.
  • Analysis of studies investigating targeting signals for chromatin remodeling complexes.
  • Synthesis of findings on the involvement of non-coding RNAs in chromatin remodeling.

Main Results:

  • Chromatin remodeling complexes utilize specific domains to recognize targeting signals like histone modifications, DNA features, and non-coding RNAs.
  • Non-coding RNAs are increasingly recognized for their roles in directing, scaffolding, and regulating chromatin remodeling enzymes.
  • Dysfunctional chromatin remodeling is implicated in various cancers.

Conclusions:

  • Precise targeting of chromatin remodelers is vital for maintaining cellular homeostasis and preventing diseases like cancer.
  • Non-coding RNA interactions represent a key mechanism for achieving specific targeting and regulation of chromatin remodelers.
  • Further research into RNA-mediated chromatin targeting could reveal new therapeutic strategies for cancer.