Coupling of Spinosad Fermentation and Separation Process via Two-Step Macroporous Resin Adsorption Method

Area of Science:

• Biotechnology
• Chemical Engineering
• Separation Science

Background:

• Spinosad is a valuable insecticide produced through fermentation.
• Conventional separation methods like crystallization can be inefficient for spinosad recovery.
• Optimizing spinosad production and purification is crucial for industrial applications.

Purpose of the Study:

• To investigate a two-step resin adsorption technology for spinosad production and separation.
• To establish an integrated process combining fermentation, adsorption, and membrane separation for spinosad.
• To enhance spinosad yield, purity, and productivity.

Main Methods:

• A two-step resin adsorption strategy was employed, with resin added during and after fermentation.
• Macroporous resin was added at 50 g/L during the early cultivation stage.
• A membrane separation technique was integrated with the resin adsorption process.

Main Results:

• Adding macroporous resin at the beginning of fermentation increased spinosad concentration by 14.45%.
• The developed two-step adsorption-membrane process achieved 95.43% purity and 87% yield for spinosad.
• These results surpassed conventional crystallization, which yielded 93.23% purity and 79.15% yield.

Conclusions:

• The two-step macroporous resin adsorption method effectively couples spinosad fermentation and separation.
• This integrated process significantly increases spinosad productivity and recovery efficiency.
• The developed technology offers a superior alternative for spinosad purification, improving both yield and purity.