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Reduced Lateral Root Branching Density Improves Drought Tolerance in Maize.

Ai Zhan1, Hannah Schneider1, Jonathan P Lynch2

  • 1State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Yangling, Shaanxi 712100, China (A.Z.); andDepartment of Plant Science, Pennsylvania State University, University Park, Pennsylvania 16802 (A.Z., H.S., J.P.L.).

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Selecting for fewer but longer lateral roots in maize (Zea mays) enhances drought tolerance by improving water acquisition. This root trait offers a promising strategy for developing more resilient crops.

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Area of Science:

  • Agricultural Science
  • Plant Biology
  • Genetics

Background:

  • Root traits influence resource acquisition and crop performance.
  • Reducing metabolic costs of soil exploration may enhance water uptake under stress.

Purpose of the Study:

  • To test if reduced lateral root branching density improves drought tolerance in maize.
  • To investigate the link between root architecture, water acquisition, and biomass production under drought.

Main Methods:

  • Maize recombinant inbred lines with contrasting lateral root phenotypes (few-long vs. many-short) were evaluated.
  • Experiments were conducted in greenhouse mesocosms and field conditions under water stress.
  • Measurements included root respiration, rooting depth, leaf water content, stomatal conductance, biomass, and yield.

Main Results:

  • Maize with few but long lateral roots exhibited reduced root respiration and deeper rooting.
  • This phenotype led to greater leaf relative water content and stomatal conductance under drought.
  • Field trials showed significantly greater shoot biomass and yield in the few-long lateral root lines under water stress.

Conclusions:

  • Reduced lateral root branching density significantly improves drought tolerance in maize.
  • The few-long lateral root phenotype is a potential selection target for enhancing crop resilience to drought.
  • This strategy may be applicable to other cereal crops for improved water acquisition.