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Calibration of 125I-polymer standards with 125I-brain paste standards for use in quantitative receptor
A J Nazarali1, J S Gutkind, J M Saavedra
1Section on Pharmacology, National Institute of Mental Health, Bethesda, MD 20892.
Abstract:
125I-Polymer standards were calibrated by interpolation of their optical densities in [125I]-brain paste standard curves to obtain dpm/mg protein. There was a linear relationship between the calibrated polymer standards and the dpm/mg polymer, as provided by the manufacturer. One dpm/mg polymer was equivalent to 7.34 +/- 0.22 dpm/mg protein. Receptor quantification in selected rat brain areas with comparison to either brain paste or calibrated polymer standards yielded similar results.
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