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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Flow Cytometry-Based Quantification and Analysis of Myocardial B-Cells

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Pulsed flows in flow analysis: Potentialities, limitations and applications.

Pablo González¹, Moisés Knochen¹, Milton K Sasaki²

¹Faculty of Chemistry, Universidad de la República, Montevideo, Uruguay.

|June 17, 2015

Summary

Pulsed flow systems, often using solenoid pumps in multi-pumping setups, offer advantages in flow analysis. These systems enhance radial mass transfer and fluid mixing for improved analytical applications.

Keywords:

Flow analysis Multi-pumping flow systems Pulsed flows Solenoid pumps

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Area of Science:

Analytical Chemistry
Chemical Engineering

Background:

Traditionally, flow analysis relied on steady, pulseless flow for sample handling reproducibility.
Peristaltic and syringe pumps dominated early flow analyzer designs.

Purpose of the Study:

To critically review pulsed flow systems in flow analysis.
To highlight the characteristics, potential, and limitations of these systems.
To emphasize the benefits of pulsed flow, including enhanced mass transfer and mixing.

Main Methods:

Review of existing literature on pulsed flow systems.
Analysis of instrumentation development for pulsed flow.
Examination of diverse analytical applications utilizing pulsed flow.

Main Results:

Increasing adoption of pulsed flow systems, particularly those employing solenoid pumps (multi-pumping flow systems).
Pulsed flow streams exhibit advantageous characteristics like high radial mass transference.
Effective fluid mixing is another key benefit of pulsed flow.

Conclusions:

Pulsed flow systems, despite historical reliance on steady flow, present significant advantages for flow analysis.
These systems offer improved sample handling and analytical performance.
Further development and application of pulsed flow technology are warranted.