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Related Experiment Video

Updated: Apr 10, 2026

Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean

Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean

Published on: December 23, 2017

Intronic promoter-mediated feedback loop regulates bean PvSR2 gene expression.

Wanning Yang¹, Xiujuan Bei¹, Meng Liu¹

¹College of Life Science, Capital Normal University, Beijing 100048, China.

Biochemical and Biophysical Research Communications

|June 17, 2015

Summary

Bean plants utilize an intronic promoter to create a transcription factor (PvMTF-1) that positively regulates its own gene (PvSR2) expression, revealing a novel gene regulation feedback loop.

Keywords:

Feedback loop regulation Intronic promoter Metal-responsive element Phaseolus vulgaris Transcription factor

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Area of Science:

Molecular Biology
Plant Gene Regulation
Transcriptional Control

Background:

Intronic promoters enhance gene expression diversity but their trans-acting mechanisms are unclear.
Bean stress-related gene 2 (PvSR2) uses an intronic promoter to produce PvMTF-1, a transcription factor.

Purpose of the Study:

Investigate the trans-acting mechanism of the intronic promoter in PvSR2 gene regulation.
Determine if PvMTF-1 regulates its host gene, PvSR2, through a metal-responsive element (MRE).

Main Methods:

DNA-protein interaction assays.
Analysis of PvMTF-1 expression in bean leaves.
Gene expression analysis.

Main Results:

PvMTF-1 was confirmed to bind to the MRE within the PvSR2 promoter.
PvMTF-1 binding up-regulates PvSR2 expression, establishing a positive feedback loop.
Demonstrated a trans-acting mechanism for intronic promoter-mediated gene regulation.

Conclusions:

Intronic promoters can regulate host gene expression in trans.
A positive regulatory feedback loop involving PvMTF-1 and PvSR2 was identified.
Findings add complexity to intron-mediated gene regulation mechanisms.