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PALM and STORM: Into large fields and high-throughput microscopy with sCMOS detectors

Pedro Almada1, Siân Culley1, Ricardo Henriques1

  • 1Quantitative Imaging and NanoBiophysics Group, MRC Laboratory for Molecular Cell Biology and Department of Cell and Developmental Biology, University College London, Gower Street, WC1E 6BT London, United Kingdom.

Methods (San Diego, Calif.)
|June 17, 2015
PubMed
Summary

Super-resolution microscopy techniques like Photo-Activation Localization Microscopy (PALM) and Stochastic Optical Reconstruction Microscopy (STORM) achieve higher resolution. Advancements in sensors and algorithms now enable high-throughput SMLM, overcoming previous speed limitations.

Keywords:
HardwareHomogenizationSingle-molecule localizationsCMOS

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