Related Experiment Video

Updated: Apr 10, 2026

Silencing of BRCA2 to Identify Novel BRCA2-regulated Biological Functions in Cultured Human Cells
09:24

Silencing of BRCA2 to Identify Novel BRCA2-regulated Biological Functions in Cultured Human Cells

Published on: August 12, 2015

9.7K

PRUNE2 is a human prostate cancer suppressor regulated by the intronic long noncoding RNA PCA3

Ahmad Salameh1, Alessandro K Lee2, Marina Cardó-Vila3

  • 1David H. Koch Center for Applied Research of Genitourinary Cancers, The University of Texas M.D. Anderson Cancer Center, Houston, TX 77030; Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center at Houston, Houston, TX 77030;

Proceedings of the National Academy of Sciences of the United States of America
|June 17, 2015
PubMed

Insights

Prostate cancer antigen 3 (PCA3) is a long noncoding RNA that regulates PRUNE2, a tumor suppressor. Their interaction offers a new target for prostate cancer diagnosis and therapy.

Area of Science:

  • Molecular Biology
  • Genetics
  • Oncology

Background:

  • Prostate cancer antigen 3 (PCA3) is a specific biomarker for prostate cancer, but its function is unknown.
  • Understanding PCA3's role is crucial for developing new diagnostic and therapeutic strategies.

Purpose of the Study:

  • To elucidate the function of PCA3 and its regulatory mechanism in prostate cancer.
  • To identify potential therapeutic targets based on the PCA3-PRUNE2 axis.

Main Methods:

  • Identification of PRUNE2 as a target gene of PCA3.
  • Investigation of the regulatory mechanism involving RNA editing by adenosine deaminase acting on RNA (ADAR).
  • Assessment of the effects of PRUNE2 and PCA3 on cell proliferation and tumor growth in vitro and in vivo.

Main Results:

  • PCA3 is classified as an antisense intronic long noncoding RNA (lncRNA) that regulates PRUNE2.
  • A novel regulatory mechanism involving a PRUNE2/PCA3 double-stranded RNA and ADAR-dependent RNA editing was identified.
  • PRUNE2 and PCA3 exhibited opposing effects on prostate cancer cell proliferation and tumor growth.
  • Coregulation and RNA editing of PRUNE2 and PCA3 were validated in human prostate cancer specimens.

Conclusions:

  • PCA3 functions as a dominant-negative oncogene, while PRUNE2 acts as a tumor suppressor in prostate cancer.
  • The PRUNE2/PCA3 regulatory axis represents a significant molecular target for prostate cancer diagnostics and therapeutics.
Keywords:
ADARPCA3PRUNE2long noncoding RNAprostate cancer

More Related Videos

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients12:13

Sequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients

Published on: November 19, 2019

7.3K
Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis07:42

Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis

Published on: November 26, 2015

14.0K

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
10.2K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

3.9K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.4K
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
7.9K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
10.2K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

3.1K