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Cloning and sequencing of the bovine gastrin gene
1University Department of Clinical Chemistry, Rigshospitalet, Copenhagen, Denmark.
Molecular Endocrinology (Baltimore, Md.)
|October 1, 1989
Abstract:
In order to deduce the primary structure of bovine preprogastrin we therefore sequenced a gastrin DNA clone isolated from a bovine liver cosmid library. Bovine preprogastrin comprises 104 amino acids and consists of a signal peptide, a 37 amino acid spacer-sequence, the gastrin-34 sequence followed by an amidation-site (Gly-Arg-Arg), and a C-terminal nonapeptide. Comparison with human, porcine, and rat cDNA sequences revealed extensive homology in the coding region as well as in short noncoding structures.