Related Experiment Video
Updated: Apr 10, 2026
Protocol for Plasmodium falciparum Infections in Mosquitoes and Infection Phenotype Determination
Published on: July 4, 2007
[Application of Nested PCR in the Diagnosis of Imported Plasmodium Ovale Infection]
Objective:
To identity Plasmodium ovale infection by 18S rRNA gene nested PCR.
Methods:
Whole blood and filter paper blood samples of malaria patients in Shandong Province were collected during 2012-2013. The parasites were observed under a microscope with Giemsa staining. The genome DNA of blood samples were extracted as PCR templates. Genus- and species-specific primers were designed according to the Plasmodium 18S rRNA gene sequences. Plasmodium ovale-positive specimens were identified by nested PCR as well as verified by sequencing.
Results:
There were 7 imported cases of P. ovale infection in the province during 2012-2013. Nested PCR results showed that the P. ovale specific band (800 bp) was amplified in all the 7 specimens. Blast results indicated that the PCR products were consistent with the Plasmodium ovale reference sequence in GenBank.
Conclusion:
Seven imported cases of ovale malaria in Shandong Province in 2012-2013 are confirmed by nested PCR.
