Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Pharmacokinetics in Geriatric Patients: Effect of Age on Drug Excretion

Pharmacokinetics in Geriatric Patients: Effect of Age on Drug Excretion

In geriatric patients, renal physiology undergoes significant changes, including diminished renal blood flow and a lower glomerular filtration rate (GFR), leading to alterations in medication clearance. Drugs such as aminoglycoside antibiotics, lithium, and digoxin, which rely on glomerular filtration for removal from the body, particularly impact pharmacokinetics. These drugs tend to have slower clearance rates in older adults, necessitating careful dosage considerations.Evaluation of renal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A new brake on podocyte injury: ATOH8 meets TGF-β.

American journal of physiology. Renal physiology·2026

Same author

Discovery and Cryo-EM-Guided Development of a Neuropilin-2-Binding Aptamer for Receptor Antagonism.

Journal of the American Chemical Society·2026

Same author

High-throughput, organ-scale 3D tubule tracking using TubuleMAP.

Research square·2026

Same author

High-throughput, organ-scale 3D tubule tracking using TubuleMAP.

bioRxiv : the preprint server for biology·2026

Same author

A single cell atlas of mouse podocytes upon injury identifies kidney zone-dependent responses.

bioRxiv : the preprint server for biology·2026

Same author

SPATIALLY PATTERNED PODOCYTE STATE TRANSITIONS COORDINATE AGING OF THE GLOMERULUS.

bioRxiv : the preprint server for biology·2026

Search research articles

Related Experiment Video

Updated: Apr 10, 2026

The Mouse Isolated Perfused Kidney Technique

The Mouse Isolated Perfused Kidney Technique

Published on: November 17, 2016

Interstitial pericytes decrease in aged mouse kidneys.

Ania Stefanska¹, Diana Eng¹, Natalya Kaverina¹

¹Division of Nephrology, Department of Medicine, University of Washington, Seattle, WA 98104, USA.

|June 18, 2015

Summary

This summary is machine-generated.

Aging kidneys lose pericytes, crucial cells supporting blood vessels. This loss contributes to vascular damage and kidney fibrosis in aged mice.

Keywords:

NG2 PDGFβ-receptor endothelium nephropathy tubulo-interstitial fibrosis

More Related Videos

3D Visualization of Retinal Vascular Pericytes in Mice by Immunostaining

3D Visualization of Retinal Vascular Pericytes in Mice by Immunostaining

Published on: November 1, 2024

Mouse Kidney Transplantation: Models of Allograft Rejection

Mouse Kidney Transplantation: Models of Allograft Rejection

Published on: October 11, 2014

Related Experiment Videos

Last Updated: Apr 10, 2026

The Mouse Isolated Perfused Kidney Technique

The Mouse Isolated Perfused Kidney Technique

Published on: November 17, 2016

3D Visualization of Retinal Vascular Pericytes in Mice by Immunostaining

3D Visualization of Retinal Vascular Pericytes in Mice by Immunostaining

Published on: November 1, 2024

Mouse Kidney Transplantation: Models of Allograft Rejection

Mouse Kidney Transplantation: Models of Allograft Rejection

Published on: October 11, 2014

Area of Science:

Nephrology
Gerontology
Vascular Biology

Background:

Kidney function declines with age due to changes in kidney structures.
Pericytes are vital for maintaining blood vessel health and function.
Age-related pericyte loss is linked to vascular damage and fibrosis.

Purpose of the Study:

To investigate the fate and role of kidney pericytes in the aging process.
To understand how pericyte changes contribute to age-related kidney vascular alterations and fibrosis.

Main Methods:

Comparative analysis of kidney tissues from young (3-month-old) and aged (27-month-old) mice.
Immunohistochemical staining for capillaries (CD31), pericytes (NG2, PDGFRβ), and fibrosis markers (collagen-I, -III, αSMA).

Main Results:

Aged kidneys exhibited a significant reduction in capillaries and associated pericytes (NG2+/PDGFRβ+), particularly in the medulla.
Pericyte-deficient capillaries were often dilated in aged kidneys.
Increased interstitial fibrosis (collagen-I/III) and a rise in pro-fibrotic pericytes (PDGFRβ+/αSMA+) were observed in aged kidneys.

Conclusions:

The decline in kidney interstitial pericytes is a key factor in age-related changes to peritubular vasculature.
Pericyte loss and their transformation into pro-fibrotic cells contribute to kidney fibrosis during aging.