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Updated: Apr 10, 2026
Generation and Culturing of Primary Human Keratinocytes from Adult Skin
Published on: December 22, 2017
[Effects of calcipotriol on the expression of S100A8 in human keratinocytes]
Dawei Wu1, Chao Wu1, Hongzhong Jin2
1Department of Dermatology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
Objective:
To explore the effects of calcipotriol on the expression of S100A8 in human keratinocytes.
Methods:
Cultured HaCaT cells were divided into 4 groups of blank without interventions, tumor necrosis factor-alpha (TNF-α) 24 h stimulation with 100 ng/ml TNF-α, calcipotriol 24 h stimulation with 10⁻⁵ -10⁻⁹ mol/L calcipotriol and calcipotriol+TNF-α 24 h stimulation. The relative expression of S100A8 mRNA was detected and calculated by real-time quantitative polymerase chain reaction (PCR).
Results:
The relative expression of S100A8 mRNA was up-regulated to (19.623 ± 3.486) folds (P < 0.01) under a 24 h stimulation of 100 ng/ml TNF-α versus blank. The expression of S100A8 was up-regulated to (5.029 ± 1.056) and (2.848 ± 0.612) folds (both P < 0.01) when cultured for 24 h with 10⁻⁷, 10⁻⁸ mol/L calcipotriol versus blank respectively. And the expression of S100A8 was down-regulated to (59.51 ± 3.31)% (P < 0.05) when cultured with 10⁻⁵ mol/L calcipotriol+TNF-α versus TNF-α-stimulated cells. The expression of S100A8 was up-regulated to (1.873 ± 0.153) folds (P < 0.01) when cultured with 10⁻⁷ mol/L calcipotriol+TNF-α versus TNF-α-stimulated cells.
Conclusions:
TNF-α induces a high expression of S100A8 in cultured human keratinocytes in vitro. Calcipotriol bi-directionally affects the expression of S100A8: A high concentration (10⁻⁵ mol/L) calcipotriol down-regulates while a low concentration (10⁻⁷ - 10⁻⁸ mol/L) calcipotriol up-regulates the expression of S100A8.
