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Published on: April 12, 2019
Bricks and blueprints: methods and standards for DNA assembly
Arturo Casini1, Marko Storch1, Geoffrey S Baldwin1
1Centre for Synthetic Biology and Innovation, Department of Life Sciences, Imperial College London, London SW7 2AZ, UK.
Abstract:
DNA assembly is a key part of constructing gene expression systems and even whole chromosomes. In the past decade, a plethora of powerful new DNA assembly methods - including Gibson Assembly, Golden Gate and ligase cycling reaction (LCR) - have been developed. In this Innovation article, we discuss these methods as well as standards such as the modular cloning (MoClo) system, GoldenBraid, modular overlap-directed assembly with linkers (MODAL) and PaperClip, which have been developed to facilitate a streamlined assembly workflow, to aid the exchange of material between research groups and to create modular reusable DNA parts.
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