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Published on: July 17, 2021
Characterization of microsatellite loci for an Australian epiphytic orchid, Dendrobium calamiforme, using Illumina
Dorset W Trapnell1, Rochelle R Beasley2, Stacey L Lance2
1Plant Biology Department, University of Georgia, Athens, Georgia 30602 USA.
Premise Of The Study:
Microsatellite loci were developed for the epiphytic pencil orchid Dendrobium calamiforme for population genetic and phylogeographic investigation of this Australian taxon.
Methods And Results:
Nineteen microsatellite loci were identified from an Illumina paired-end shotgun library of D. calamiforme. Polymorphism and genetic diversity were assessed in 24 individuals from five populations separated by a maximum distance of ∼80 km. All loci were polymorphic with two to 14 alleles per locus, expected heterozygosity ranging from 0.486 to 0.902, and probability of identity values ranging from 0.018 to 0.380.
Conclusions:
These novel markers will serve as valuable tools for investigation of levels of genetic diversity as well as patterns of gene flow, genetic structure, and phylogeographic history.
