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Development and characterization of microsatellite markers for Veratrum maackii (Melanthiaceae)
Yue Li1, Satoshi Iijima2, Masayuki Maki1
1Botanical Gardens, Tohoku University, Aoba, Sendai 980-0862, Japan.
Premise Of The Study:
Microsatellite markers were developed in Veratrum maackii (Melanthiaceae) to assess the pattern of population genetic structures across the species' distribution.
Methods And Results:
Ten polymorphic loci were isolated from V. maackii using 454 shotgun pyrosequencing and the biotin capture method. The number of alleles per locus ranged from three to 19. The observed heterozygosity and expected heterozygosity ranged from 0.143 to 0.929 and 0.269 to 0.904, respectively.
Conclusions:
These newly developed microsatellite markers are useful for assessing the genetic diversity, population structure, and demographic history of V. maackii across its distribution range.
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