Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genome Copying Errors02:46

Genome Copying Errors

5.6K
DNA replication is a well-evolved process that copies millions of base pairs with high fidelity during each cell division. Occasionally a wrong base or a long stretch of wrong bases may get added to the daughter strands. If the errors are left unchecked, cells might accumulate several mutations that might endanger their  survival. Therefore, the copying errors are checked and repaired at three levels.
5.6K
Sanger Sequencing01:57

Sanger Sequencing

779.7K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
779.7K
Detection of Gross Error: The Q Test01:00

Detection of Gross Error: The Q Test

7.9K
When one or more data points appear far from the rest of the data, there is a need to determine whether they are outliers and whether they should be eliminated from the data set to ensure an accurate representation of the measured value. In many cases, outliers arise from gross errors (or human errors) and do not accurately reflect the underlying phenomenon. In some cases, however, these apparent outliers reflect true phenomenological differences. In these cases, we can use statistical methods...
7.9K
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

4.7K
4.7K
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

16.4K
The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
16.4K
Next-generation Sequencing03:00

Next-generation Sequencing

101.8K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
101.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Efficient evidence-based genome annotation with EviAnn.

Nature methods·2026
Same author

Genomic and physiological changes in a sexually selected and frugivorous bird radiation.

Current biology : CB·2026
Same author

Ambiguity in identifying parameters of an SIR model when fitting epidemic incidence data.

Mathematical biosciences and engineering : MBE·2026
Same author

Efficient evidence-based genome annotation with EviAnn.

bioRxiv : the preprint server for biology·2025
Same author

Laminar chaotic saddle within a turbulent attractor.

Physical review. E·2024
Same author

k-nonical space: sketching with reverse complements.

Bioinformatics (Oxford, England)·2024

Related Experiment Video

Updated: Apr 9, 2026

Rare Event Detection Using Error-corrected DNA and RNA Sequencing
10:36

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

12.7K

QuorUM: An Error Corrector for Illumina Reads.

Guillaume Marçais1, James A Yorke1, Aleksey Zimin1

  • 1IPST, University of Maryland, College Park, MD, USA.

Plos One
|June 18, 2015
PubMed
Summary

QuorUM software corrects errors in Illumina sequencing reads, improving genome assembly quality. This tool enhances downstream analysis by reducing errors and increasing contig length, making it efficient for large datasets.

Area of Science:

  • Genomics
  • Bioinformatics
  • Computational Biology

Background:

  • Illumina sequencing generates high-coverage, short reads with a low but significant error rate.
  • Sequencing errors introduce erroneous k-mers, complicating downstream genomic analyses like assembly.
  • Error correction is crucial for improving the utility of short-read sequencing data.

Purpose of the Study:

  • To develop and evaluate QuorUM, a novel software for error correcting Illumina sequencing reads.
  • To minimize erroneous k-mers while preserving true k-mers for improved genome assembly.
  • To assess QuorUM's performance against existing error correction tools.

Main Methods:

  • Developed QuorUM software based on minimizing distinct erroneous k-mers and preserving true k-mers.

More Related Videos

Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples
07:30

Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples

Published on: June 8, 2020

13.0K
Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER
14:06

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER

Published on: June 23, 2012

15.9K

Related Experiment Videos

Last Updated: Apr 9, 2026

Rare Event Detection Using Error-corrected DNA and RNA Sequencing
10:36

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

12.7K
Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples
07:30

Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples

Published on: June 8, 2020

13.0K
Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER
14:06

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER

Published on: June 23, 2012

15.9K
  • Introduced a composite statistic, π, to measure error correction success.
  • Evaluated QuorUM using actual Illumina reads from reference genomes.

    • Main Results:

    • QuorUM produces trimmed and error-corrected reads, leading to assemblies with longer contigs and fewer errors.
    • QuorUM outperforms several published error correctors across various metrics.
    • QuorUM demonstrates efficient performance on large datasets (1 billion bases/day/core) and significantly improves SOAPdenovo assembly (1.1-4x N50 increase).

    Conclusions:

    • QuorUM effectively corrects Illumina sequencing errors, enhancing genome assembly quality.
    • The software is efficient, scalable, and provides superior performance compared to existing methods.
    • QuorUM is available as a standalone package or MaSuRCA module under GPL.