Related Experiment Video
Updated: Apr 9, 2026

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
Published on: December 23, 2022
Environmental phosphate differentially affects virulence phenotypes of uropathogenic Escherichia coli isolates
M Grillo-Puertas1, M G Martínez-Zamora1, M R Rintoul1
1a Instituto Superior de Investigaciones Biológicas , INSIBIO (CONICET-UNT) and Instituto de Química Biológica "Dr Bernabé Bloj", Facultad de Bioquímica, Química y Farmacia (UNT) ; Tucumán , Argentina.
Abstract:
K-12 Escherichia coli cells grown in static media containing a critical phosphate (Pi) concentration ≥25 mM maintained a high polyphosphate (polyP) level in stationary phase, impairing biofilm formation, a phenomenon that is triggered by polyP degradation. Pi concentration in human urine fluctuates according to health state. Here, the influence of environmental Pi concentration on the occurrence of virulence traits in uropathogenic E. coli (UPEC) isolated from acute prostatitis patients was evaluated. After a first screening, 3 isolates were selected according to differential biofilm formation profiles depending on media Pi concentration. For each isolate, biofilm positive and negative conditions were established. Regardless of the isolate, biofilm formation capacity was accompanied with curli and cellulose production and expression of some key virulence factors associated with adhesion. When the selected isolates were grown in their non-biofilm-forming condition, low concentrations of nalidixic acid and ciprofloxacin induced biofilm formation. Interestingly, similar to laboratory strains, polyP degradation induced biofilm formation in the selected isolates. Data demonstrated the complexity of UPEC responses to environmental Pi and the importance of polyP metabolism in the virulence of clinical isolates.
Related Concept Videos
Bacterial Gastroenteritis
Regulation of Bacterial Virulence
Stringent Response in E. coli
Microbiota of the Urogenital Tract
Gene Regulation in Microbial Communities: Quorum Sensing
Evolution of New Traits in Microbes

