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HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis

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[Researching on fingerprint of Inulacappa by HPLC]

Di-fei-fei Xiong, Di Zhu, Dan Tan

Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica

|June 19, 2015

Abstract

Objective:

This study is to establish the fingerprint and find out the common chromatographic peaks of Inula cappa by HPLC.

Method:

The HPLC analysis was performed on an Agilent Eclipse Plus C18 column (2.1 mm x 150 mm, 1.8 μm) with 0.1% fomic acid aqueous solution-0.1% fomic acid acetonitrile solution as mobile phase at a flow rate of 0.3 · mL(-1) · min(-1); The detective wavelength is 325 nm; The column temperature is 45 °C.

Result:

The results indicated that 5 of 17 common peaks were identified . The similarity about 10 groups of Inulacappais is over 0.95.

Conclusion:

This method is able to be a scientific basis of quality assessment according to its convenient and reliable.

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