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Some properties of bovine mitochondrial serine tRNA gene transcript synthesized with T7 RNA polymerase
1Department of Life Chemistry, Graduate School at Nagatsuta, Tokyo Institute of Technology, Yokohama, Japan.
Nucleic Acids Symposium Series
|January 1, 1989
Abstract:
Bovine mitochondrial serine tRNA(AGY) gene transcript was synthesized in vitro with T7 RNA polymerase, and it was capable of being aminoacylated with mitochondrial serine tRNA synthetase. The melting profiles of the transcript was similar to those of native serine tRNA(AGY), suggesting that the higher-order structure of the transcript does not much differ from that of native serine tRNA(AGY). Several transcripts with base-substitution were also constructed and their aminoacylation capacity was investigated.