Related Experiment Video
Updated: Apr 9, 2026
An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function
Published on: May 4, 2021
Experimental Model to Study the Role of Retinoblastoma Gene Product (pRb) for Determination of Adipocyte
B V Popov1, P S Shilo, O V Zhidkova
1Institute of Cytology, Russian Academy of Science, St. Petersburg, Russia, borisvp478@gmail.com.
Abstract:
Using stable constitutive expression of retinoblastoma gene product (pRb) in polypotent mesenchymal 10T1/2 cells we obtained stable cell lines hyperexpressing functionally active or inactive mutant pRb. The cells producing active exogenous pRb demonstrated high sensitivity to adipocyte differentiation inductors, whereas production of inactive form of the exogenous protein suppressed adipocyte differentiation. The obtained lines can serve as the experimental model for studying the role of pRb in determination of adipocyte differentiation.
