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Detection of T-Cadherin Expression in Mouse Embryos.
K A Rubina1, V A Smutova1, M L Semenova2
1Department of Biochemistry and Molecular Medicine, Faculty of Fundamental Medicine, Lomonosov Moscow State University, Lomonosovskiy Prosp., 31/5, 119192, Moscow, Russia.
Acta Naturae
|June 19, 2015
Summary
T-cadherin is expressed in the developing mouse brain and heart during early embryogenesis. This finding suggests T-cadherin may play a role in blood vessel formation.
Area of Science:
- Developmental Biology
- Molecular Biology
- Genetics
Background:
- T-cadherin is a cell surface protein with known roles in various biological processes.
- Understanding its expression patterns during embryonic development is crucial for elucidating its functions.
Purpose of the Study:
- To investigate the spatiotemporal expression of T-cadherin during early mouse embryogenesis.
- To identify specific developmental stages and tissues where T-cadherin is present.
Main Methods:
- In situ hybridization was employed to detect T-cadherin mRNA.
- Immunofluorescent staining of whole mouse embryos was performed.
- Confocal microscopy was utilized for high-resolution imaging.
Main Results:
- T-cadherin expression was observed in the developing mouse brain by embryonic day 8.75 (E8.75).
- T-cadherin expression was detected in the developing mouse heart by embryonic day 11.5 (E11.5).
Conclusions:
- T-cadherin expression is initiated early in specific embryonic tissues, including the brain and heart.
- The expression pattern suggests a potential role for T-cadherin in vascular development during embryogenesis.
