Search research articles

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Liposomal lipid nanoparticles containing dihydrosphingomyelin exhibit improved stability and extended hepatic and extrahepatic transfection.

Journal of controlled release : official journal of the Controlled Release Society·2026

Same author

Direct Counting of mRNA Copies Inside Individual Lipid Nanoparticles Using In Situ Lysis and Labeling.

Analytical chemistry·2026

Same author

Hybrid Lipid Nanoparticles Containing Iron Oxide Nanoparticles and Doxorubicin.

Langmuir : the ACS journal of surfaces and colloids·2026

Same author

Formulation and administration timing of lipid derivatized JAK-inhibitor and corticosteroid modulate saRNA-LNP inflammation and expression.

Journal of controlled release : official journal of the Controlled Release Society·2026

Same author

Silencing of PAI-1 using siRNA-lipid nanoparticles reduces thrombosis and prolongs life span in murine models.

Blood·2026

Same author

Islet amyloid disrupts MHC class II antigen presentation and delays autoimmune diabetes in NOD mice.

Diabetologia·2025

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Apr 9, 2026

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Published on: February 25, 2021

Microfluidic Mixing: A General Method for Encapsulating Macromolecules in Lipid Nanoparticle Systems.

Alex K K Leung¹, Yuen Yi C Tam¹, Sam Chen¹

¹Department of Biochemistry and Molecular Biology, The University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3.

The Journal of Physical Chemistry. B

|June 19, 2015

Summary

Microfluidic mixing efficiently produces lipid nanoparticles (LNP) with tunable structures for small interfering RNA (siRNA). This technique also successfully encapsulates larger molecules like mRNA and DNA, and even gold nanoparticles.

More Related Videos

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

Published on: March 3, 2020

Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles

Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles

Published on: January 7, 2019

Related Experiment Videos

Last Updated: Apr 9, 2026

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Published on: February 25, 2021

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

Published on: March 3, 2020

Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles

Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles

Published on: January 7, 2019

Area of Science:

Biotechnology
Materials Science
Nanotechnology

Background:

Lipid nanoparticles (LNP) are crucial for delivering nucleic acids.
Microfluidic mixing is an efficient method for LNP manufacturing.
Previous studies indicate nanostructured cores in LNP-siRNA systems.

Purpose of the Study:

To investigate how lipid composition affects LNP-siRNA structure.
To determine if microfluidic mixing can encapsulate macromolecules larger than siRNA.
To demonstrate the versatility of microfluidic mixing for nanoparticle encapsulation.

Main Methods:

Microfluidic mixing for LNP production.
Cryo-transmission electron microscopy (cryo-TEM) for structural analysis.
Molecular simulations for structural prediction.

Main Results:

LNP-siRNA structure transitions towards a bilayer as DSPC content increases.
Microfluidic mixing successfully encapsulated mRNA (1.7 kb) and plasmid DNA (6 kb).
Negatively charged gold nanoparticles (5 nm) were encapsulated, forming a 'currant bun' morphology.

Conclusions:

LNP nanostructure is tunable via lipid composition, offering a continuum of structures.
Microfluidic mixing is a versatile technique for encapsulating diverse molecules and nanoparticles within LNPs.
The 'currant bun' morphology of gold-containing LNP is consistent with predicted LNP-siRNA structures.