Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Efficacy, safety, and dose selection of efepoetin alfa in anemic patients on maintenance dialysis: a phase 2, randomized, multicenter, active-controlled trial.

Kidney research and clinical practice·2026
Same author

cP1P Maintains Long-Term Pluripotency in Human Pluripotent Stem Cells.

International journal of stem cells·2026
Same author

Cell-free protein synthesis systems for post-translational modifications.

Progress in molecular biology and translational science·2026
Same author

Green Synthesis and Characterization of Silver Nanoparticles Using Traditional Medicinal Herb Phyllanthus Maderaspatensis for their Antibacterial and Anti-biofilm Activities.

Applied biochemistry and biotechnology·2026
Same author

A Multikinase Inhibitor AX-0085 Blocks FGFR1 Activation to Overcomes Osimertinib Resistance in Non-Small Cell Lung Cancer.

Biomedicines·2026
Same author

New frontiers and applications of cell-free systems.

Progress in molecular biology and translational science·2026

Related Experiment Video

Updated: Apr 9, 2026

Genome Editing with CompoZr Custom Zinc Finger Nucleases ZFNs
09:11

Genome Editing with CompoZr Custom Zinc Finger Nucleases ZFNs

Published on: June 14, 2012

26.3K

Recent developments and clinical studies utilizing engineered zinc finger nuclease technology.

Young-Il Jo1, Hyongbum Kim2,3, Suresh Ramakrishna4

  • 1Brandeis University, Waltham, MA, USA.

Cellular and Molecular Life Sciences : CMLS
|June 20, 2015
PubMed
Summary

Zinc finger nucleases (ZFNs) offer efficient targeted genome editing for gene function research and disease therapies. Recent advancements significantly enhance ZFN activity and specificity, expanding their applications in medicine and biotechnology.

Keywords:
Farm animalsPre-clinical trialsProgrammable nucleasesTargeted genetic modificationsTherapeutic applicationsZFN architectureZFN deliveryZFN modification

More Related Videos

Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells
12:13

Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells

Published on: August 23, 2014

11.3K
Mouse Genome Engineering Using Designer Nucleases
12:04

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

29.4K

Related Experiment Videos

Last Updated: Apr 9, 2026

Genome Editing with CompoZr Custom Zinc Finger Nucleases ZFNs
09:11

Genome Editing with CompoZr Custom Zinc Finger Nucleases ZFNs

Published on: June 14, 2012

26.3K
Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells
12:13

Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells

Published on: August 23, 2014

11.3K
Mouse Genome Engineering Using Designer Nucleases
12:04

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

29.4K

Area of Science:

  • Molecular Biology
  • Genetic Engineering
  • Biotechnology

Background:

  • Homologous recombination for targeted genetic modification is inefficient.
  • Zinc finger nucleases (ZFNs) are engineered nucleases for precise DNA cleavage.
  • ZFNs enable targeted gene corrections, additions, knockouts, and structural variations.

Purpose of the Study:

  • To review the developments and future perspectives of ZFN technology.
  • To highlight advancements improving ZFN activity and specificity.
  • To discuss the clinical applications of ZFN-mediated genome editing.

Main Methods:

  • Modification of DNA-binding and FokI cleavage domains to enhance ZFNs.
  • Optimization of cell culture methods, including cold shock and small molecules.
  • Enrichment of ZFN-induced mutant cells using episomal surrogate reporters.

Main Results:

  • Significant enhancement of ZFN activity and specificity through domain modifications.
  • Increased ZFN efficiency via improved culture techniques and stability enhancers.
  • Successful application of ZFNs in ongoing human clinical studies.

Conclusions:

  • ZFN technology has undergone substantial improvements over 17 years.
  • Enhanced ZFNs are powerful tools for genetic research and therapeutic development.
  • ZFN-mediated genome editing shows significant promise in medicine and biotechnology.