Related Experiment Video
Updated: Apr 9, 2026
Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
Mass Spectrometry-Based Proteomic Study Makes High-Density Lipoprotein a Biomarker for Atherosclerotic Vascular
Chiz-Tzung Chang1, Chao-Yuh Yang2, Fuu-Jen Tsai3
1College of Medicine, China Medical University, 91 Hsueh-Shih Road, Taichung 40402, Taiwan ; Division of Nephrology, China Medical University Hospital, 2 Yu-Der Road, Taichung 40447, Taiwan ; L5 Research Center, China Medical University Hospital, Taichung, Taiwan.
Insights
High-density lipoprotein (HDL) dysfunction contributes to atherosclerosis and reduced survival. Mass spectrometry-based proteomics offers a powerful method to analyze HDL composition and modifications in disease states.
Area of Science:
- Biochemistry
- Proteomics
- Cardiovascular Disease Research
Background:
- High-density lipoprotein (HDL) is a complex of lipids and proteins crucial for cardiovascular health.
- HDL dysfunction is implicated in atherosclerosis development and reduced patient survival.
- Understanding HDL composition and modifications is vital for disease insights.
Purpose of the Study:
- To review advancements in mass spectrometry (MS)-based quantitative proteomic techniques for HDL analysis.
- To explore the role of HDL proteomics and lipoprotein modifications in various diseases.
- To discuss the significance of HDL subfractionation studies in understanding disease mechanisms.
Main Methods:
- Utilizing mass spectrometry (MS)-based proteomics for high-throughput analysis of HDL proteins.
- Applying HDL subfractionation techniques based on size, charge, or composition.
- Reviewing recent developments in quantitative proteomic methodologies for HDL.
Main Results:
- MS-based proteomics enables detailed analysis of HDL composition and modifications.
- Subfractionation of HDL allows for targeted investigation of specific lipoprotein roles in disease.
- Recent advancements enhance the throughput and accuracy of HDL proteomic studies.
Conclusions:
- MS-based proteomics is a key technology for advancing HDL research in disease.
- Further exploration of HDL subfractions and modifications using proteomics is warranted.
- This review highlights future directions for MS-based HDL proteomics.
Abstract:
High-density lipoprotein (HDL) is a lipid and protein complex that consists of apolipoproteins and lower level HDL-associated enzymes. HDL dysfunction is a factor in atherosclerosis and decreases patient survival. Mass spectrometry- (MS-) based proteomics provides a high throughput approach for analyzing the composition and modifications of complex HDL proteins in diseases. HDL can be separated according to size, surface charge, electronegativity, or apoprotein composition. MS-based proteomics on subfractionated HDL then allows investigation of lipoprotein roles in diseases. Herein, we review recent developments in MS-based quantitative proteomic techniques, HDL proteomics and lipoprotein modifications in diseases, and HDL subfractionation studies. We also discuss future directions and perspectives in MS-based proteomics on HDL.
Related Concept Videos
01:25Blood Studies for Cardiovascular System III: Serum Lipid Profile
Serum lipids are fats and fatty substances in the blood and are crucial for various bodily functions, including energy storage, cellular structure, and hormone production. Serum lipids consist of cholesterol, triglycerides, and phospholipids.
Cholesterol is a soft, fat-like substance found in all body cells. It is crucial for producing hormones, vitamin D, and substances that aid...
01:30Atherosclerosis I: Introduction