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Related Concept Videos

Supercritical Fluid Chromatography01:18

Supercritical Fluid Chromatography

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Supercritical fluid chromatography (SFC) provides a beneficial substitute for gas chromatography (GC) and liquid chromatography (LC) for certain samples because it merges the top attributes of both techniques. SFC allows the separation and analysis of compounds that GC or LC does not easily manage. These compounds are traditionally nonvolatile or thermally unstable, making GC unsuitable and lacking functional groups required for HPLC analysis.
SFC utilizes a supercritical fluid mobile phase,...
1.3K

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Lipidomics by Supercritical Fluid Chromatography.

Laurent Laboureur1, Mario Ollero2,3, David Touboul4

  • 1CNRS, Institut de Chimie des Substances Naturelles, UPR2301, Université Paris-Sud, Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex, France. laurent.laboureur@cnrs.fr.

International Journal of Molecular Sciences
|June 20, 2015
PubMed
Summary

Supercritical fluid chromatography (SFC) offers a powerful technique for lipid analysis, compatible with various detectors. This review explores its resurgence and application across diverse lipid classes.

Keywords:
lipidmass spectrometrysupercritical fluid chromatography

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Area of Science:

  • Analytical Chemistry
  • Lipidomics
  • Chromatography

Background:

  • Supercritical fluid chromatography (SFC) experienced a decline in popularity but has regained interest with new instrument advancements.
  • SFC is compatible with organic solvent extracts, making it suitable for complex lipid samples.

Purpose of the Study:

  • To review the role and applications of SFC in lipid analysis.
  • To discuss the advantages and disadvantages of SFC for various lipid classes.

Main Methods:

  • Review of historical and current applications of SFC in lipid analysis.
  • Discussion of SFC compatibility with universal and specific detection methods (UV, ELS, MS).
  • Analysis of SFC performance for different lipid categories (fatty acyls, glycerolipids, etc.).

Main Results:

  • SFC is a versatile technique for separating diverse lipid classes.
  • The technique's compatibility with mass spectrometry enables detailed lipid profiling.
  • SFC offers distinct advantages for specific lipid analyses.

Conclusions:

  • SFC is a valuable and re-emerging tool for comprehensive lipid analysis.
  • The technique's suitability for various lipid classes warrants its continued application in lipidomics.
  • Further exploration of SFC in lipid research is encouraged.