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DNA Damage can Stall the Cell Cycle

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

DNA Damage Can Stall the Cell Cycle

DNA Damage Can Stall the Cell Cycle

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Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.

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Related Experiment Video

Updated: Apr 9, 2026

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

Bifurcation in Cell Cycle Dynamics Regulated by p53.

Md Jahoor Alam¹, Sanjay Kumar², Vikram Singh³

¹School of Computational and Integrative Sciences, Jawaharlal Nehru University, New Delhi-110067, India; College of Applied Medical Sciences, University of Hail, Hail-2440, Kingdom of Saudi Arabia.

|June 20, 2015

Summary

Irradiation impacts p53 dynamics, causing oscillations or stabilization. The cell cycle

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Yeast As a Chassis for Developing Functional Assays to Study Human P53

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Area of Science:

* Molecular and Systems Biology
* Biophysics
* Mathematical Biology

Background:

* The p53 protein is a critical regulator of cellular responses to stress, including DNA damage.
* Cell cycle dynamics are complex and involve intricate feedback loops between molecular species.
* Understanding the interplay between p53 and cell cycle networks is crucial for comprehending cellular fate decisions.

Purpose of the Study:

* To investigate the regulatory mechanism of p53 on cell cycle dynamics under irradiation stress.
* To model the interactions between p53 and cell cycle networks.
* To analyze the impact of irradiation strength on p53 and cell cycle molecular species.

Main Methods:

* Development of a mathematical model simulating interacting p53 and cell cycle networks.
* Analysis of p53 dynamics under varying irradiation strengths.
* Examination of cell cycle molecular species (MPF and cyclin) dynamics through bifurcation analysis.

Main Results:

* Irradiation induces distinct phases in p53 dynamics: oscillating and oscillation death (stabilized).
* The collapse time of p53 oscillations (Δt) is dependent on irradiation strength.
* Cell cycle molecular species exhibit diverse states (oscillation death, sustained oscillations, chaos) influenced by p53-mediated irradiation stress.

Conclusions:

* A critical threshold (Δtc) for irradiation-induced p53 dynamics exists.
* Below this threshold, the cell cycle may return to normal; above it, cell cycle arrest (apoptosis) is predicted.
* The study provides insights into p53's role in determining cell fate following DNA damage.