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Evaluation of the PREVI® Isola automated seeder system compared to reference manual inoculation for antibiotic

S Le Page1, A van Belkum, C Fulchiron

  • 1URMITE UM 63 CNRS 7278 IRD 198 INSERM U1905, IHU Méditerranée Infection, Faculté de Médecine et de Pharmacie, Aix-Marseille Université, 27 Boulevard Jean Moulin, 13385, Marseille CEDEX 05, France.

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
|June 21, 2015
PubMed

Insights

The PREVI® Isola automated seeder system offers a faster, reproducible method for antibiotic susceptibility testing (AST) using the disk diffusion (DD) technique. This automated system demonstrates excellent accuracy and efficiency compared to manual DD testing.

Area of Science:

  • Clinical Microbiology
  • Antimicrobial Resistance
  • Diagnostic Technology

Background:

  • Disk diffusion (DD) is a widely used manual method for antibiotic susceptibility testing (AST) due to its simplicity and low cost.
  • Automated microdilution systems, while common, can be less sensitive in detecting specific resistance mechanisms.
  • There is a need for efficient and reliable automated methods for DD testing in clinical laboratories.

Purpose of the Study:

  • To evaluate the PREVI® Isola automated seeder system with a new protocol for DD testing.
  • To compare the performance of the automated system against the manual DD reference method.
  • To assess the time efficiency, reproducibility, and accuracy of the automated DD protocol.

Main Methods:

  • The PREVI® Isola system was used with a novel protocol involving eight bacterial suspension deposits and two rotation rounds.
  • Performance was evaluated against manual DD reference testing using clinical and reference bacterial strains.
  • Reproducibility, repeatability, and correlation were assessed using European Committee on Antimicrobial Susceptibility Testing (EUCAST) guidelines.

Main Results:

  • The automated protocol significantly reduced seeding time to 51 seconds per plate (70 plates/hour).
  • Reproducibility and repeatability were excellent, showing no statistical discrimination compared to manual methods.
  • An excellent correlation (coefficient 0.977) was observed between automated and manual DD testing across 3,528 zones of inhibition.
  • The new PREVI® Isola protocol achieved 99% sensitivity and 100% specificity compared to manual DD.

Conclusions:

  • The PREVI® Isola automated seeder system, using the new protocol, provides an efficient and accurate alternative for disk diffusion antibiotic susceptibility testing.
  • The system offers excellent reproducibility and high correlation with the manual reference method.
  • This automated approach meets EUCAST recommendations for DD interpretation, enhancing laboratory workflow.

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