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Published on: August 8, 2018
Characterization of the bay scallop (Argopecten irradians concentricus Say) transcriptome and identification of
Sigang Fan1, Dongling Zhang2, Yihui Guo1
1Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.
Abstract:
The bay scallop Argopecten irradians concentricus is a commercially important bivalve species for aquaculture in China. However, the scarcity of transcripotomic and genomic resources of the bay scallop has hindered the progress of genetic improvement of the animal. To change this situation, de novo transcriptome sequencing with Illumina HiSeq™2000 sequencing platform was carried out for the bay scallop. Through de novo assembly, 107,145 high quality unigenes were obtained. Totally, 44,901 and 34,673 unigenes were annotated in the Nr database and Swiss-Prot database, respectively. With gene ontology (GO) annotations, 77,834 (51.58%), 49,367 (32.72%) and 23,695 (15.70%) were involved in biological processes, cellular components and molecular functions, respectively. One hundred and thirty seven unigenes putatively involved in growth were identified through blastx. More than 2706 simple sequence repeats (SSRs) and 82,116 single nucleotide polymorphisms (SNPs) were also detected. These genes and markers may serve as new valuable resources for functional genomic and genetic studies on the scallop A. i. concentricus.
