Related Experiment Video
Updated: Apr 9, 2026

Standardization of a Cytometric Bead Assay Based on Egg-Yolk Antibodies
Published on: May 19, 2023
Population data of the GlobalFiler(®) Express loci in South Portuguese population
Carina Almeida1, Teresa Ribeiro2, Ana Rita Oliveira1
1National Institute of Legal Medicine and Forensic Sciences, Portugal; University of Lisbon, Faculty of Sciences, Portugal.
Abstract:
Allele frequencies and other relevant forensic parameters for 21 loci studied with GlobalFiler(®) Express amplification kit (Life Technologies) were calculated in a population of individuals residing in the south of Portugal. Blood stain samples were obtained from a total of 502 unrelated individuals involved in paternity testing casework and directly PCR amplified with GlobalFiler(®) Express following manufacturer's instructions. This kit comprises all the loci included in the extended European Standard Set (ESS) and in the Combined DNA Index System (CODIS), besides the very polymorphic D2S441, D19S433, and SE33. In our laboratory this is used as a screening tool to solve complex cases, as fatherless paternity tests or to help in paternity investigations where there is the need to study additional genetic markers. These studies are necessary to calculate statistical forensic parameters, such as power of discrimination or as power of exclusion. Statistical parameters including heterozigosity, homozigosity and combined power of exclusion were estimated.
Related Concept Videos
Selected Data About Geographic Locations
Gene Flow
Distributions to Estimate Population Parameter
What is Population Genetics?
Hardy-Weinberg Principle
Single Nucleotide Polymorphisms-SNPs

