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3D print customized sample holders for live light sheet microscopy.

Emeric Jeandupeux1, Valérie Lobjois1, Bernard Ducommun2

  • 1Université de Toulouse, ITAV-USR3505, F-31106 Toulouse, France; CNRS, ITAV-USR3505, F-31106 Toulouse, France.

Biochemical and Biophysical Research Communications
|June 21, 2015
PubMed
Summary
This summary is machine-generated.

Researchers developed a 3D-printed hydrogel casting kit for versatile light sheet microscopy sample holders. This customizable solution facilitates live imaging of multiple biological specimens, including tumor spheroids.

Keywords:
3D printingCell biologyLight sheet imagingMicroscopySPIMSample holder

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Area of Science:

  • Biomedical Engineering
  • Microscopy
  • Biotechnology

Background:

  • Widespread adoption of light sheet microscopy is hindered by the absence of adaptable, non-intrusive sample holders for live imaging diverse biological samples.
  • Existing sample holders often lack versatility and customization, limiting their utility across various research applications.

Purpose of the Study:

  • To address the limitations of current sample holders for light sheet microscopy.
  • To introduce a novel, customizable sample holder fabrication method using 3D printing.
  • To enable versatile and non-intrusive live imaging of biological specimens.

Main Methods:

  • Application of 3D printing technology to design and fabricate a customizable casting kit.
  • Utilized the casting kit to prepare hydrogel sample holders tailored to specific shapes and specimen numbers.
  • Demonstrated the system's efficacy using a four-sample holder for parallel live monitoring of multicellular tumor spheroid growth.

Main Results:

  • Successfully fabricated versatile and customizable hydrogel sample holders using a 3D-printed casting kit.
  • The developed sample holders are adaptable to various biological sample configurations and sizes.
  • Enabled parallel live imaging of multicellular tumor spheroids, showcasing the system's practical application.

Conclusions:

  • 3D printing offers a facile and adaptable approach to creating custom sample holders for light sheet microscopy.
  • This solution overcomes previous limitations, enhancing the versatility and accessibility of live imaging techniques.
  • All necessary design files are publicly available, promoting wider adoption within the scientific community.