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Published on: April 2, 2017
Protective Effect of Micronized Purified Flavonoid Fraction on Ischemia/Reperfusion Injury of Rat Liver
1Department of General Surgery, Gaziantep University School of Medicine, Gaziantep, Turkey.
Background:
Flavonoids have been subjected to considerable investigation because of its antioxidant and anti-inflammatory properties. However, there is no previously reported study about its effect on hepatic ischemia/reperfusion (I/R). We investigated the effects of micronized purified flavonoid fraction (MPFF) on hepatic I/R injury in rats.
Methods:
Thirty rats were recruited in the study as follows: group A, sham operation (n = 10); group B, I/R (n = 10); and group C, I/R+MPFF (n = 10). In group C, rats received (80 mg/kg/day) MPFF by gavage for 3 days before surgery, 30 minutes before ischemia and just before the reperfusion. Blood samples were taken, and serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH) levels were measured to assess liver functions. Liver tissues were taken for histological evaluation and to determine the total antioxidant capacity (TAC), catalase (CAT), total oxidant status (TOS), oxidative stress index (OSI), and myeloperoxidase (MPO).
Results:
The present data showed a decrease in AST, ALT, and LDH levels in the MPFF-treated rats when compared with I/R group rats (P < .001 for all). In the MPFF-treated rats, tissue levels of TOS, OSI, and MPO were significantly lower than those in the I/R group (P < .01, P < .001, and P < .05, respectively). Increases in TAC and CAT levels were statistically significant in the MPFF-treated rats compared with the I/R group (P = .01 for both). On the other hand, MPFF attenuated histological alterations that were induced by I/R.
Conclusions:
The present study demonstrates that MPFF ameliorates I/R-induced liver damage, probably through antioxidant and anti-inflammatory properties.
Insights
Micronized purified flavonoid fraction (MPFF) protects against liver injury caused by ischemia/reperfusion (I/R) in rats. MPFF demonstrated significant antioxidant and anti-inflammatory effects, reducing liver damage markers.
Area of Science:
- Hepatology
- Pharmacology
- Biochemistry
Background:
- Flavonoids are known for antioxidant and anti-inflammatory properties.
- Hepatic ischemia/reperfusion (I/R) injury lacks specific treatment studies involving flavonoids.
- This study investigates the protective effects of micronized purified flavonoid fraction (MPFF) on hepatic I/R injury in a rat model.
Purpose of the Study:
- To evaluate the efficacy of MPFF in mitigating liver damage induced by I/R.
- To assess the biochemical and histological changes in the liver following I/R and MPFF treatment.
- To explore the potential antioxidant and anti-inflammatory mechanisms of MPFF in hepatic I/R.
Main Methods:
- A rat model of hepatic I/R was established.
- Thirty rats were divided into three groups: sham operation, I/R, and I/R + MPFF.
- MPFF was administered orally to the treatment group before and during the I/R procedure. Serum liver enzymes (AST, ALT, LDH) and liver tissue markers (TAC, CAT, TOS, OSI, MPO) were analyzed. Histological evaluation was performed.
Main Results:
- MPFF treatment significantly reduced serum AST, ALT, and LDH levels compared to the I/R group.
- MPFF administration led to a significant decrease in liver tissue oxidative stress markers (TOS, OSI, MPO) and a significant increase in antioxidant markers (TAC, CAT).
- Histological examination revealed that MPFF attenuated the I/R-induced liver tissue damage.
Conclusions:
- MPFF effectively ameliorates hepatic I/R injury in rats.
- The protective effects of MPFF are likely mediated by its antioxidant and anti-inflammatory properties.
- This study suggests MPFF as a potential therapeutic agent for managing I/R-induced liver damage.
