Related Experiment Video
Updated: Apr 9, 2026

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock
Published on: June 29, 2017
Effective production of low crystallinity Poly(3-hydroxybutyrate) by recombinant E. coli strain JM109 using crude
Mohan Ganesh1, Arivazhagan Senthamarai1, Santhanam Shanmughapriya1
1Medical Microbiology Laboratory, Department of Microbiology, Centre for Excellence in Life Sciences, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India.
Abstract:
Utilization of bio-diesel by-products (glycerol) for microbial polymer production has created a novel biorefinery concept. In the present study, recombinant Escherichia coli JM109 was used for the production of P(3 HB) from glycerol as carbon source. Batch fermentation in a 7.5L bioreactor with the statistically optimized culture condition (pre-treated glycerol: 27.5 g/L and casein hydrolysate: 5.25 g/L) scaled up the P3HB production to 65% (∼ 8 g/L). FTIR, (1)H and (13)C NMR analysis proved the polymer produced to be P(3 HB). Gel permeation chromatography, Differential Scanning Calorimetry (DSC) and thermogravimetric analysis (TGA) demonstrated the produced P(3 HB) to have high molecular weight (2.84 × 10(6)) and lowered crystallinity (∼ 30%) compared to commercial polymer. Integrating the production efficiency and the thermal characteristics of the polymer produced by recombinant E. coli, the viability and sustainability of biofuels and biopolymers for economic human need could be enhanced.
Related Concept Videos
Production of Organic Acids
Production of Pharmaceuticals
Bioplastics
Biosynthesis of Polysaccharides
Biosynthesis in Bacteria

