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Quantification of hypoglycin A in serum using aTRAQ(®) assay.

François Boemer¹, Michelle Deberg¹, Roland Schoos¹

¹Biochemical Genetics Laboratory, Human Genetics, CHU Liege, University of Liege, Belgium.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

|June 22, 2015

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A new method accurately quantifies hypoglycin A, the cause of atypical myopathy (AM) in horses. This reliable detection in equine blood confirms AM and aids understanding of the toxin

Area of Science:

Veterinary Toxicology
Equine Medicine
Analytical Chemistry

Background:

Hypoglycin A is the identified causative agent of atypical myopathy (AM) in horses.
Accurate identification and quantification of hypoglycin A in equine biological fluids are crucial for diagnosing AM and understanding intoxication mechanisms.

Purpose of the Study:

To develop and validate a reliable method for quantifying hypoglycin A in equine biological fluids.
To confirm the presence of hypoglycin A in horses diagnosed with atypical myopathy.

Main Methods:

Quantification of hypoglycin A using the aTRAQ kit for amino acid analysis.
Optimization of mass spectrometry acquisition to detect hypoglycin A-specific MRM transitions.

Main Results:

Keywords:

Amino acids Atypical myopathy Hypoglycin A Mass spectrometry aTRAQ

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The developed method demonstrated high accuracy and linearity (0.09–50 μmol/L) with excellent precision (CV < 8%).
Significant hypoglycin A concentrations were detected in 55 samples from 25 confirmed AM horses.
Hypoglycin A was not detected in serum samples from 8 healthy control horses.

Conclusions:

The aTRAQ variant method for hypoglycin A quantification has been analytically and clinically validated.
This reliable approach is effective for the diagnosis and study of atypical myopathy in horses.