Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Light Acquisition02:16

Light Acquisition

9.9K
In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.
9.9K
Photoreceptors and Plant Responses to Light02:00

Photoreceptors and Plant Responses to Light

29.1K
Light plays a significant role in regulating the growth and development of plants. In addition to providing energy for photosynthesis, light provides other important cues to regulate a range of developmental and physiological responses in plants.
29.1K
UV–Vis Spectroscopy of Conjugated Systems01:32

UV–Vis Spectroscopy of Conjugated Systems

9.2K
Organic compounds with conjugated double bonds show strong absorption features in the UV–visible region of the electromagnetic spectrum attributed to π → π* electronic excitations. Generally, a UV–vis absorption spectrum is recorded as a plot of absorbance vs wavelength. The wavelength of maximum absorbance, which manifests as a peak in the absorption spectrum, is denoted as λmax.
One of the factors influencing λmax is the extent of conjugation in...
9.2K
Light as Energy01:35

Light as Energy

98.8K
The energy required to carry out photosynthesis is light— typically electromagnetic radiation from the sun. The range of all possible wavelengths is known as the electromagnetic spectrum.
Photons
A photon is a discrete electromagnetic particle or bundle of energy. Photons are characterized by their frequency, wavelength, and amplitude, similar to the properties of a wave. Waves with higher frequencies transmit more energy and have shorter wavelengths than longer wavelengths that transmit...
98.8K
IR and UV–Vis Spectroscopy of Aldehydes and Ketones01:29

IR and UV–Vis Spectroscopy of Aldehydes and Ketones

8.0K
Infrared spectroscopy, also known as vibrational spectroscopy, is mainly used to determine the types of bonds and functional groups in molecules. In aldehydes and ketones, the carbonyl (C=O) bond shows an absorption around 1710 cm-1. The C=O bond vibration of an aldehyde occurs at lower frequencies than that of a ketone. In addition to the C=O absorption in an aldehyde, the aldehydic C–H bond also gives two peaks in the 2700–2800 cm-1 range. This absorption, coupled with the...
8.0K
UV–Vis Spectroscopy: Woodward–Fieser Rules01:29

UV–Vis Spectroscopy: Woodward–Fieser Rules

29.8K
UV–Visible absorption spectra of conjugated dienes arise from the lowest energy π → π* transitions. The light-absorbing part of the molecule is called the chromophore, and the substituents directly attached to the chromophore are called auxochromes. A strong correlation exists between the absorption maxima, λmax, and the structure of a conjugated π system. The Woodward–Fieser rules predict the value of λmax for a given structure by adding the...
29.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Collagen 1-mediated CXCL1 secretion in tumor cells activates fibroblasts to promote radioresistance of esophageal cancer.

Cell reports·2026
Same author

National Trends in Radical Hysterectomy at Gynecologic Oncology Fellowship Training Programs.

O&G open·2026
Same author

Impact of Male Human Papillomavirus (HPV) Vaccination on HPV Infection and HPV-Related Diseases: A Systematic Review.

Reviews in medical virology·2026
Same author

Alcohol consumption and oral human papillomavirus infection among men living with HIV: a cross-sectional study from the ULACNet 201 trial.

Scientific reports·2026
Same author

Genotype distribution of human papillomavirus (HPV) types in oral gargle specimens among men living with HIV in Mexico, Brazil, and Puerto Rico: A cross-sectional study.

The Journal of infection·2026
Same author

Recent national trends in pelvic and paraaortic lymphadenectomy in gynecologic oncology fellowship training programs: ACGME data from 2019-2025.

Gynecologic oncology reports·2026

Related Experiment Video

Updated: Apr 9, 2026

Evaluation of Photosynthetic Behaviors by Simultaneous Measurements of Leaf Reflectance and Chlorophyll Fluorescence Analyses
10:20

Evaluation of Photosynthetic Behaviors by Simultaneous Measurements of Leaf Reflectance and Chlorophyll Fluorescence Analyses

Published on: August 9, 2019

13.4K

Chlorophyll content retrieval from hyperspectral remote sensing imagery.

Xiguang Yang1, Ying Yu, Wenyi Fan

  • 1Northeast Forestry University, 26 Hexing rd., 150040, Harbin, China, yangxiguang21@163.com.

Environmental Monitoring and Assessment
|June 23, 2015
PubMed
Summary

Accurate forest chlorophyll estimation using hyperspectral remote sensing is vital for assessing forest health. This study developed a stepwise model to retrieve leaf and canopy chlorophyll content from Hyperion imagery, aiding in forest growth and disease monitoring.

More Related Videos

RGB and Spectral Root Imaging for Plant Phenotyping and Physiological Research: Experimental Setup and Imaging Protocols
11:37

RGB and Spectral Root Imaging for Plant Phenotyping and Physiological Research: Experimental Setup and Imaging Protocols

Published on: August 8, 2017

17.3K
Applying Hyperspectral Reflectance Imaging to Investigate the Palettes and the Techniques of Painters
07:05

Applying Hyperspectral Reflectance Imaging to Investigate the Palettes and the Techniques of Painters

Published on: June 18, 2021

2.9K

Related Experiment Videos

Last Updated: Apr 9, 2026

Evaluation of Photosynthetic Behaviors by Simultaneous Measurements of Leaf Reflectance and Chlorophyll Fluorescence Analyses
10:20

Evaluation of Photosynthetic Behaviors by Simultaneous Measurements of Leaf Reflectance and Chlorophyll Fluorescence Analyses

Published on: August 9, 2019

13.4K
RGB and Spectral Root Imaging for Plant Phenotyping and Physiological Research: Experimental Setup and Imaging Protocols
11:37

RGB and Spectral Root Imaging for Plant Phenotyping and Physiological Research: Experimental Setup and Imaging Protocols

Published on: August 8, 2017

17.3K
Applying Hyperspectral Reflectance Imaging to Investigate the Palettes and the Techniques of Painters
07:05

Applying Hyperspectral Reflectance Imaging to Investigate the Palettes and the Techniques of Painters

Published on: June 18, 2021

2.9K

Area of Science:

  • Ecology
  • Remote Sensing
  • Forestry

Background:

  • Chlorophyll content is crucial for photosynthesis and influences leaf spectral properties.
  • Accurate forest canopy chlorophyll estimation is essential for monitoring forest health, growth, and disease.
  • Hyperspectral remote sensing offers high spatial resolution for detailed chlorophyll content retrieval.

Purpose of the Study:

  • To develop a stepwise method for retrieving forest canopy chlorophyll content using hyperspectral imagery.
  • To establish a leaf chlorophyll inversion model and a pixel reflectance to leaf reflectance conversion model.
  • To estimate canopy chlorophyll content per unit ground surface area for forest health assessment.

Main Methods:

  • Analysis of leaf spectral curves and identification of 25 spectral characteristic parameters.
  • Development of a leaf chlorophyll content inversion model using stepwise regression.
  • Application of a geometrical-optical model (4-scale) for pixel to leaf reflectance conversion, with key parameters (M0, PT, PG) estimated using leaf area index (LAI).

Main Results:

  • A stepwise regression model successfully retrieved leaf chlorophyll content with an RMSE of 7.3574 μg/cm².
  • The multiple scattering factor (M0), probability of viewing sunlit tree crown (PT), and background (PG) were logarithmically related to LAI (R² > 0.9).
  • Canopy chlorophyll content per unit ground surface area was accurately estimated using leaf chlorophyll content and LAI.

Conclusions:

  • Hyperspectral remote sensing, combined with a stepwise inversion model, provides an effective method for estimating forest canopy chlorophyll content.
  • The developed models enable accurate mapping of chlorophyll content, supporting forest growth stage assessment and disease detection.
  • This approach offers a valuable tool for ecological and forestry applications requiring detailed forest health monitoring.