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Microwave-assisted Functionalization of Polyethylene glycol and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation

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PEGylation to Improve Protein Stability During Melt Processing.

Parker Lee¹, Jenna Towslee¹, João Maia¹

¹Department of Macromolecular Science and Engineering, Case Western Reserve University, Cleveland, Ohio, 44106, USA.

Macromolecular Bioscience

|June 23, 2015

Summary

PEGylation enhances protein stability during melt processing, improving lysozyme activity and dispersion. This method offers a promising solution for biopharmaceutical delivery systems, reducing activity loss and modifying drug release profiles.

Keywords:

PEGylation melt processing poly(lactic-co-glycolic acid)protein stability

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Area of Science:

Biopharmaceutical drug delivery
Polymer science
Protein stabilization

Background:

Biopharmaceuticals are effective but face delivery challenges.
Melt processing offers solvent-free, high-efficiency protein encapsulation.
Protein activity loss during melt processing due to heat and shear is a significant issue.

Purpose of the Study:

To investigate the impact of poly(ethylene glycol) or PEGylation on protein stability during melt processing.
To evaluate the effect of PEGylation on lysozyme activity and dispersion within poly(lactic-co-glycolic acid) or PLGA.
To assess PEGylation's influence on drug release kinetics in melt-processed systems.

Main Methods:

Utilized melt processing techniques for protein encapsulation.
Employed lysozyme as a model protein and PLGA as the matrix material.
Investigated the effects of covalent attachment of PEG (PEGylation) on protein properties.

Main Results:

PEGylation significantly increased the retained activity of lysozyme after melt processing.
Enhanced dispersion of lysozyme within the PLGA melt was observed with PEGylation.
The biphasic drug release profile typically seen in melt-processed systems was reduced by PEGylation.

Conclusions:

PEGylation is an effective strategy to enhance protein stability and activity during melt processing.
PEGylated proteins exhibit improved dispersion and modified release characteristics in PLGA-based systems.
This approach holds potential for developing more robust and effective biopharmaceutical delivery formulations.