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Related Concept Videos

Proteomics01:33

Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics
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Quantitative proteomics using SILAC: Principles, applications, and developments.

Xiulan Chen1, Shasha Wei1, Yanlong Ji1,2

  • 1Key Laboratory of Protein and Peptide Pharmaceuticals and Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing, P. R. China.

Proteomics
|June 23, 2015
PubMed
Summary
This summary is machine-generated.

Stable Isotope Labeling by Amino acids in Cell culture (SILAC) offers accurate proteome quantification. This review covers SILAC principles, diverse applications in biological research, and its current status.

Keywords:
MSQuantitative proteomicsSILACSuper-SILACTechnology

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Area of Science:

  • Proteomics
  • Biochemistry
  • Cell Biology

Background:

  • Quantitative proteomics is crucial for understanding cellular processes.
  • Existing methods like chemical labeling and label-free quantification have limitations.
  • Stable Isotope Labeling by Amino acids in Cell culture (SILAC) provides a robust alternative.

Purpose of the Study:

  • To review the fundamental principles of SILAC technology.
  • To highlight the broad applications of SILAC in biological research.
  • To discuss the current advancements and state of SILAC.

Main Methods:

  • SILAC involves the direct incorporation of stable isotope-labeled amino acids into cell culture media.
  • This method facilitates precise quantitative analysis of the cellular proteome.
  • It enables comparative proteomic studies with high accuracy and reproducibility.

Main Results:

  • SILAC offers superior quantitative accuracy and reproducibility compared to other labeling strategies.
  • It has been successfully applied to analyze proteomic changes, protein PTM dynamics, protein interactions, and protein turnover.
  • The technology is favored for its straightforward implementation in proteomic research.

Conclusions:

  • SILAC is a powerful and versatile tool for quantitative proteomic analysis.
  • Its advantages in accuracy, reproducibility, and ease of use support its widespread adoption.
  • Continued advancements promise further expansion of SILAC's utility in biological discovery.